Detection and full genome characterization of two beta CoV viruses related to Middle East respiratory syndrome from bats in Italy
Identifieur interne : 000E29 ( Main/Exploration ); précédent : 000E28; suivant : 000E30Detection and full genome characterization of two beta CoV viruses related to Middle East respiratory syndrome from bats in Italy
Auteurs : Ana Moreno [Italie] ; Davide Lelli [Italie] ; Luca De Sabato [Italie] ; Guendalina Zaccaria [Italie] ; Arianna Boni [Italie] ; Enrica Sozzi [Italie] ; Alice Prosperi [Italie] ; Antonio Lavazza [Italie] ; Eleonora Cella [Italie] ; Maria Rita Castrucci [Italie] ; Massimo Ciccozzi [Italie] ; Gabriele Vaccari [Italie]Source :
- Virology Journal [ 1743-422X ] ; 2017.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Analyse de séquence d'ARN, Animaux, Chiroptera (virologie), Coronavirus du syndrome respiratoire du Moyen-Orient (), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Glycoprotéine de spicule des coronavirus (), Glycoprotéine de spicule des coronavirus (génétique), Génome viral (génétique), Italie, Motifs et domaines d'intéraction protéique, Phylogénie, Réaction de polymérisation en chaîne, Structure tertiaire des protéines (génétique), Séquence d'acides aminés, Séquence nucléotidique.
- MESH :
- génétique : ARN viral, Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus, Génome viral, Structure tertiaire des protéines.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Chiroptera.
- Analyse de séquence d'ARN, Animaux, Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus, Italie, Motifs et domaines d'intéraction protéique, Phylogénie, Réaction de polymérisation en chaîne, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Chiroptera (virology), Genome, Viral (genetics), Italy, Middle East Respiratory Syndrome Coronavirus (chemistry), Middle East Respiratory Syndrome Coronavirus (classification), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Phylogeny, Polymerase Chain Reaction, Protein Interaction Domains and Motifs, Protein Structure, Tertiary (genetics), RNA, Viral (genetics), Sequence Analysis, RNA, Spike Glycoprotein, Coronavirus (chemistry), Spike Glycoprotein, Coronavirus (genetics).
- MESH :
- chemical , chemistry : Spike Glycoprotein, Coronavirus.
- chemical , genetics : RNA, Viral, Spike Glycoprotein, Coronavirus.
- chemistry : Middle East Respiratory Syndrome Coronavirus.
- classification : Middle East Respiratory Syndrome Coronavirus.
- genetics : Genome, Viral, Middle East Respiratory Syndrome Coronavirus, Protein Structure, Tertiary.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- virology : Chiroptera.
- Amino Acid Sequence, Animals, Base Sequence, Italy, Phylogeny, Polymerase Chain Reaction, Protein Interaction Domains and Motifs, Sequence Analysis, RNA.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV), which belongs to beta group of coronavirus, can infect multiple host species and causes severe diseases in humans. Multiple surveillance and phylogenetic studies suggest a bat origin. In this study, we describe the detection and full genome characterization of two CoVs closely related to MERS-CoV from two Italian bats,
Pool of viscera were tested by a pan-coronavirus RT-PCR. Virus isolation was attempted by inoculation in different cell lines. Full genome sequencing was performed using the Ion Torrent platform and phylogenetic trees were performed using IQtree software. Similarity plots of CoV clade c genomes were generated by using SSE v1.2. The three dimensional macromolecular structure (3DMMS) of the receptor binding domain (RBD) in the S protein was predicted by sequence-homology method using the protein data bank (PDB).
Both samples resulted positive to the pan-coronavirus RT-PCR (IT-batCoVs) and their genome organization showed identical pattern of MERS CoV. Phylogenetic analysis showed a monophyletic group placed in the Beta2c clade formed by MERS-CoV sequences originating from humans and camels and bat-related sequences from Africa, Italy and China. The comparison of the secondary and 3DMMS of the RBD of IT-batCoVs with MERS, HKU4 and HKU5 bat sequences showed two aa deletions located in a region corresponding to the external subdomain of MERS-RBD in IT-batCoV and HKU5 RBDs.
This study reported two beta CoVs closely related to MERS that were obtained from two bats belonging to two commonly recorded species in Italy (
Url:
DOI: 10.1186/s12985-017-0907-1
PubMed: 29258555
PubMed Central: 5735805
Affiliations:
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Le document en format XML
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Nutrition and Veterinary Public Health,</institution></institution-wrap>Viale Regina Elena 299, 00161 Rome, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Viale Regina Elena 299, 00161 Rome</wicri:regionArea><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000000121622106</institution-id><institution-id institution-id-type="GRID">grid.8509.4</institution-id><institution>Dept. of Sciences,</institution><institution>University Roma Tre,</institution></institution-wrap>Viale Guglielmo Marconi 446, 00146 Rome, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Viale Guglielmo Marconi 446, 00146 Rome</wicri:regionArea><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation></author><author><name sortKey="Zaccaria, Guendalina" sort="Zaccaria, Guendalina" uniqKey="Zaccaria G" first="Guendalina" last="Zaccaria">Guendalina Zaccaria</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 9120 6856</institution-id><institution-id institution-id-type="GRID">grid.416651.1</institution-id><institution>Department of Food Safety,</institution><institution>Istituto Superiore di Sanità. Nutrition and Veterinary Public Health,</institution></institution-wrap>Viale Regina Elena 299, 00161 Rome, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Viale Regina Elena 299, 00161 Rome</wicri:regionArea><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation></author><author><name sortKey="Boni, Arianna" sort="Boni, Arianna" uniqKey="Boni A" first="Arianna" last="Boni">Arianna Boni</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 9120 6856</institution-id><institution-id institution-id-type="GRID">grid.416651.1</institution-id><institution>Department of Food Safety,</institution><institution>Istituto Superiore di Sanità. Nutrition and Veterinary Public Health,</institution></institution-wrap>Viale Regina Elena 299, 00161 Rome, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Viale Regina Elena 299, 00161 Rome</wicri:regionArea><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation></author><author><name sortKey="Sozzi, Enrica" sort="Sozzi, Enrica" uniqKey="Sozzi E" first="Enrica" last="Sozzi">Enrica Sozzi</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124 Brescia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124 Brescia</wicri:regionArea><wicri:noRegion>25124 Brescia</wicri:noRegion></affiliation></author><author><name sortKey="Prosperi, Alice" sort="Prosperi, Alice" uniqKey="Prosperi A" first="Alice" last="Prosperi">Alice Prosperi</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124 Brescia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124 Brescia</wicri:regionArea><wicri:noRegion>25124 Brescia</wicri:noRegion></affiliation></author><author><name sortKey="Lavazza, Antonio" sort="Lavazza, Antonio" uniqKey="Lavazza A" first="Antonio" last="Lavazza">Antonio Lavazza</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124 Brescia, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124 Brescia</wicri:regionArea><wicri:noRegion>25124 Brescia</wicri:noRegion></affiliation></author><author><name sortKey="Cella, Eleonora" sort="Cella, Eleonora" uniqKey="Cella E" first="Eleonora" last="Cella">Eleonora Cella</name><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1757 5329</institution-id><institution-id institution-id-type="GRID">grid.9657.d</institution-id><institution>University Campus Bio-Medico of Rome,</institution></institution-wrap>Via Álvaro del Portillo, 21, 00128 Rome, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Via Álvaro del Portillo, 21, 00128 Rome</wicri:regionArea><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation></author><author><name sortKey="Castrucci, Maria Rita" sort="Castrucci, Maria Rita" uniqKey="Castrucci M" first="Maria Rita" last="Castrucci">Maria Rita Castrucci</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 9120 6856</institution-id><institution-id institution-id-type="GRID">grid.416651.1</institution-id><institution>Department of Food Safety,</institution><institution>Istituto Superiore di Sanità. Nutrition and Veterinary Public Health,</institution></institution-wrap>Viale Regina Elena 299, 00161 Rome, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Viale Regina Elena 299, 00161 Rome</wicri:regionArea><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation></author><author><name sortKey="Ciccozzi, Massimo" sort="Ciccozzi, Massimo" uniqKey="Ciccozzi M" first="Massimo" last="Ciccozzi">Massimo Ciccozzi</name><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1757 5329</institution-id><institution-id institution-id-type="GRID">grid.9657.d</institution-id><institution>University Campus Bio-Medico of Rome,</institution></institution-wrap>Via Álvaro del Portillo, 21, 00128 Rome, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Via Álvaro del Portillo, 21, 00128 Rome</wicri:regionArea><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation></author><author><name sortKey="Vaccari, Gabriele" sort="Vaccari, Gabriele" uniqKey="Vaccari G" first="Gabriele" last="Vaccari">Gabriele Vaccari</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 9120 6856</institution-id><institution-id institution-id-type="GRID">grid.416651.1</institution-id><institution>Department of Food Safety,</institution><institution>Istituto Superiore di Sanità. Nutrition and Veterinary Public Health,</institution></institution-wrap>Viale Regina Elena 299, 00161 Rome, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Viale Regina Elena 299, 00161 Rome</wicri:regionArea><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation></author></analytic><series><title level="j">Virology Journal</title><idno type="eISSN">1743-422X</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Chiroptera (virology)</term><term>Genome, Viral (genetics)</term><term>Italy</term><term>Middle East Respiratory Syndrome Coronavirus (chemistry)</term><term>Middle East Respiratory Syndrome Coronavirus (classification)</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>Protein Interaction Domains and Motifs</term><term>Protein Structure, Tertiary (genetics)</term><term>RNA, Viral (genetics)</term><term>Sequence Analysis, RNA</term><term>Spike Glycoprotein, Coronavirus (chemistry)</term><term>Spike Glycoprotein, Coronavirus (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>Analyse de séquence d'ARN</term><term>Animaux</term><term>Chiroptera (virologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Glycoprotéine de spicule des coronavirus ()</term><term>Glycoprotéine de spicule des coronavirus (génétique)</term><term>Génome viral (génétique)</term><term>Italie</term><term>Motifs et domaines d'intéraction protéique</term><term>Phylogénie</term><term>Réaction de polymérisation en chaîne</term><term>Structure tertiaire des protéines (génétique)</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genome, Viral</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>Protein Structure, Tertiary</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term><term>Génome viral</term><term>Structure tertiaire des protéines</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chiroptera</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Italy</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>Protein Interaction Domains and Motifs</term><term>Sequence Analysis, RNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ARN</term><term>Animaux</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term><term>Italie</term><term>Motifs et domaines d'intéraction protéique</term><term>Phylogénie</term><term>Réaction de polymérisation en chaîne</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p id="Par1">Middle East respiratory syndrome coronavirus (MERS-CoV), which belongs to beta group of coronavirus, can infect multiple host species and causes severe diseases in humans. Multiple surveillance and phylogenetic studies suggest a bat origin. In this study, we describe the detection and full genome characterization of two CoVs closely related to MERS-CoV from two Italian bats, <italic>Pipistrellus kuhlii</italic> and Hypsugo savii.</p></sec><sec><title>Methods</title><p id="Par2">Pool of viscera were tested by a pan-coronavirus RT-PCR. Virus isolation was attempted by inoculation in different cell lines. Full genome sequencing was performed using the Ion Torrent platform and phylogenetic trees were performed using IQtree software. Similarity plots of CoV clade c genomes were generated by using SSE v1.2. The three dimensional macromolecular structure (3DMMS) of the receptor binding domain (RBD) in the S protein was predicted by sequence-homology method using the protein data bank (PDB).</p></sec><sec><title>Results</title><p id="Par3">Both samples resulted positive to the pan-coronavirus RT-PCR (IT-batCoVs) and their genome organization showed identical pattern of MERS CoV. Phylogenetic analysis showed a monophyletic group placed in the Beta2c clade formed by MERS-CoV sequences originating from humans and camels and bat-related sequences from Africa, Italy and China. The comparison of the secondary and 3DMMS of the RBD of IT-batCoVs with MERS, HKU4 and HKU5 bat sequences showed two aa deletions located in a region corresponding to the external subdomain of MERS-RBD in IT-batCoV and HKU5 RBDs.</p></sec><sec><title>Conclusions</title><p id="Par4">This study reported two beta CoVs closely related to MERS that were obtained from two bats belonging to two commonly recorded species in Italy (<italic>P. kuhlii</italic> and H. savii). The analysis of the RBD showed similar structure in IT-batCoVs and HKU5 respect to HKU4 sequences. Since the RBD domain of HKU4 but not HKU5 can bind to the human DPP4 receptor for MERS-CoV, it is possible to suggest also for IT-batCoVs the absence of DPP4-binding potential. More surveillance studies are needed to better investigate the potential intermediate hosts that may play a role in the interspecies transmission of known and currently unknown coronaviruses with particular attention to the S protein and the receptor specificity and binding affinity.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Su, S" uniqKey="Su S">S Su</name></author><author><name sortKey="Wong, G" uniqKey="Wong G">G Wong</name></author><author><name sortKey="Shi, W" uniqKey="Shi W">W Shi</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name></author><author><name sortKey="Lai, Ack" uniqKey="Lai A">ACK Lai</name></author><author><name sortKey="Zhou, J" uniqKey="Zhou J">J Zhou</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Wit, E" uniqKey="Wit E">E Wit</name></author><author><name sortKey="Van Doremalen, N" uniqKey="Van Doremalen N">N van 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uniqKey="O Hea T">TJ O’Shea</name></author><author><name sortKey="Cryan, Pm" uniqKey="Cryan P">PM Cryan</name></author><author><name sortKey="Cunningham, Aa" uniqKey="Cunningham A">AA Cunningham</name></author><author><name sortKey="Fooks, Ar" uniqKey="Fooks A">AR Fooks</name></author><author><name sortKey="Hayman, Dts" uniqKey="Hayman D">DTS Hayman</name></author><author><name sortKey="Luis, Ad" uniqKey="Luis A">AD Luis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brook, Ce" uniqKey="Brook C">CE Brook</name></author><author><name sortKey="Dobson, Ap" uniqKey="Dobson A">AP Dobson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Raj, Vs" uniqKey="Raj V">VS Raj</name></author><author><name sortKey="Farag, Eaba" uniqKey="Farag E">EABA Farag</name></author><author><name sortKey="Reusken, Cbem" uniqKey="Reusken C">CBEM Reusken</name></author><author><name sortKey="Lamers, Mm" uniqKey="Lamers M">MM Lamers</name></author><author><name sortKey="Pas, Sd" uniqKey="Pas S">SD Pas</name></author><author><name sortKey="Voermans, J" uniqKey="Voermans J">J Voermans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Adney, Dr" uniqKey="Adney D">DR Adney</name></author><author><name sortKey="Van Doremalen, N" uniqKey="Van Doremalen N">N van Doremalen</name></author><author><name sortKey="Brown, Vr" uniqKey="Brown V">VR Brown</name></author><author><name sortKey="Bushmaker, T" uniqKey="Bushmaker T">T Bushmaker</name></author><author><name sortKey="Scott, D" uniqKey="Scott D">D Scott</name></author><author><name sortKey="De Wit, E" uniqKey="De Wit E">E de Wit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Russo, D" uniqKey="Russo D">D Russo</name></author><author><name sortKey="Jones, G" uniqKey="Jones G">G Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ancillotto, L" uniqKey="Ancillotto L">L Ancillotto</name></author><author><name sortKey="Santini, L" uniqKey="Santini L">L Santini</name></author><author><name sortKey="Ranc, N" uniqKey="Ranc N">N Ranc</name></author><author><name sortKey="Maiorano, L" uniqKey="Maiorano L">L Maiorano</name></author><author><name sortKey="Russo, D" uniqKey="Russo D">D Russo</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Italie</li></country><region><li>Latium</li></region><settlement><li>Rome</li></settlement></list><tree><country name="Italie"><noRegion><name sortKey="Moreno, Ana" sort="Moreno, Ana" uniqKey="Moreno A" first="Ana" last="Moreno">Ana Moreno</name></noRegion><name sortKey="Boni, Arianna" sort="Boni, Arianna" uniqKey="Boni A" first="Arianna" last="Boni">Arianna Boni</name><name sortKey="Castrucci, Maria Rita" sort="Castrucci, Maria Rita" uniqKey="Castrucci M" first="Maria Rita" last="Castrucci">Maria Rita Castrucci</name><name sortKey="Cella, Eleonora" sort="Cella, Eleonora" uniqKey="Cella E" first="Eleonora" last="Cella">Eleonora Cella</name><name sortKey="Ciccozzi, Massimo" sort="Ciccozzi, Massimo" uniqKey="Ciccozzi M" first="Massimo" last="Ciccozzi">Massimo Ciccozzi</name><name sortKey="De Sabato, Luca" sort="De Sabato, Luca" uniqKey="De Sabato L" first="Luca" last="De Sabato">Luca De Sabato</name><name sortKey="De Sabato, Luca" sort="De Sabato, Luca" uniqKey="De Sabato L" first="Luca" last="De Sabato">Luca De Sabato</name><name sortKey="Lavazza, Antonio" sort="Lavazza, Antonio" uniqKey="Lavazza A" first="Antonio" last="Lavazza">Antonio Lavazza</name><name sortKey="Lelli, Davide" sort="Lelli, Davide" uniqKey="Lelli D" first="Davide" last="Lelli">Davide Lelli</name><name sortKey="Prosperi, Alice" sort="Prosperi, Alice" uniqKey="Prosperi A" first="Alice" last="Prosperi">Alice Prosperi</name><name sortKey="Sozzi, Enrica" sort="Sozzi, Enrica" uniqKey="Sozzi E" first="Enrica" last="Sozzi">Enrica Sozzi</name><name sortKey="Vaccari, Gabriele" sort="Vaccari, Gabriele" uniqKey="Vaccari G" first="Gabriele" last="Vaccari">Gabriele Vaccari</name><name sortKey="Zaccaria, Guendalina" sort="Zaccaria, Guendalina" uniqKey="Zaccaria G" first="Guendalina" last="Zaccaria">Guendalina Zaccaria</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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